Structural characterization of an Ascaris myoglobin.
نویسندگان
چکیده
منابع مشابه
Temperature-dependent Structural Perturbation of Tuna Myoglobin
To unveil the mechanism of fast autooxidation of fish myoglobins, the effect of temperature on the structural change of tuna myoglobin was investigated. Purified myoglobin was subjected to preincubation at 5, 20, 50 and 40C. Overall helical structural decay through thermal treatment up to 95C was monitored by circular dichroism spectrometry, while the structural changes around the heme pocket w...
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To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis re...
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It has been reported that crystalline human myoglobin can be resolved by electrophoresis on paper into three components (1,2). Two of these possess nearly identical absorption spectra between 500 and 600 rnp and give the same reactions with oxygen. Although it was suggested that these components differ structurally (2), no evidence has been presented to support this view. In the present study t...
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Ultraviolet photodissociation (UVPD) mass spectrometry is employed to investigate the structure of holo-myoglobin as well as its apo form transferred to the gas phase by native electrospray. UVPD provided insight into the stability of native structural elements of holo-myoglobin. The fragmentation yields from UVPD showed the greatest overall correlation with B-factors generated from the crystal...
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The myoglobin of the polychaete annelid Ophelia bicornis was isolated, purified to homogeneity and characterized. The primary structure, obtained from cDNA and protein sequencing, consists of 139 amino acid residues. The alignment with other globin sequences showed that O. bicornis myoglobin misses the pre-A helix and the first six residues of the A helix. The presence of a PheB10-GlnE7 haem di...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)43794-5